EMBA program Essay

It has been my desire for several years now to obtain a masters degree in business. To satisfy my desire for success, I intend to continue my education now. With the drive, determination, and discipline to both work at my career and attend an EMBA program, I am excited to pursue a high quality EMBA at Texas A&M Mays business school. Interested in acquiring the skill set, knowledge and business acumen necessary to become a successful manager, the Mays business program will prove central to my future success. As a Project Manager, I developed leadership skills and improved my problem-solving ability, but I never had the opportunity to take courses exploring business strategy, finance, or market forces. To realize my career goal, I clearly must enhance my abilities in these as well as other areas of business. I believe that the management core at Mays will provide me with the necessary knowledge to enhance and develop my capabilities. Also, in order to become a successful manager, it is imperative that I gain a more thorough education in foreign markets, business technology, and competition. As a manager in the twenty-first century, the ability to understand business on a global scale will be highly prized. Moreover, I look forward to interacting with the highly qualified, diverse students of the Mays EMBA program. With the small class sections, the program ensures the intimacy necessary to learn from each other’s varying perspectives and backgrounds. I know I could both contribute and grow in this unique environment where all students are fully employed. From my time as a software consultant and as a project manager at a global company, I have both developed strong team abilities and have seen the value and synergies of combining people with different backgrounds, knowledge, and experience levels. I believe that entering your EMBA program will provide me with the career possibilities and exposure to resources, such as networks and learning tools, which I do not currently have. At Mays, I know that my investment of time, energy, and money will be well rewarded. At this point in my life, I believe I have great potential to gain much from a business degree since I have been exposed to the business environment for the past ten years. I now posses a strong foundation to build upon and I am ready to assume the rigors and challenges of the Mays EMBA program. Of the two business schools to which I am applying, the Mays school is my first choice. Apart from their excellent curriculum, Mays school is very conveniently located to me. Please contact me if you need any clarifications or have questions regarding my qualifications or application.

Different Types of Groups

The concept of the social group is people coming together and having a sense of identity because of a common space, action, or any other things that can bind together people. There is several identified social groups: primary groups, secondary groups, collective groups, in-groups, and out-groups. The primary group is one that shares close and lifelong personal relationships, wherein each member shares personal space and lives and has close relations and interactions.Family and close friends are examples of primary groups, as their members are binded by strong personal ties. A sports group or an academic group that is strongly linked emotionally and physically can also be considered as a primary group. The primary groups shapes and influences a person’s identity. The secondary group relationship is characterized by being formal and temporary, instead of being closely personal and lifelong. Performance of functions is what links together people in the secondary group, and the re lationship is more fleeting and less personal.An example would be a temporary co-worker in the office, who one is constantly in contact with, but has no strong personal ties. A collective group on the other hand is one that is bonded together by a common goal or an interest that links its members. The collective group does similar things and its members have similar interests. For instance, a church group or an environmental group is an example of a collective group. The in-group is the group where the individual feels that he/she belongs in; it is where one has loyalty to or respect to.Members of the in-group shares a similar identity, like families or people of a certain ethnicity or race. Conversely, the out-group is the social group that a person is not inclined to relate, even disdains or is opposed to such group. For instance, a conservative man despising people from other races – the out-group. The different types of groups refer to the personal circles that an individ ual belongs to. These are product of his/her social environment and socialization, which then directs and determines membership to a certain group.

Influence of Advertisement on Brand Preference Essay

Vithya Vivekananthan, Faculty of Commerce and Management, Eastern University, Vantharumoolai, Sri Lanka vcvithya@gmail. com ABSTRACT Measuring the influence of Advertisement in Consumer Brand Preference is very essential for every marketer. If advertisement does not create any positive change in consumers’ brand preference, all the resources such as money, time and efforts spent on advertisement will go in vain. Most of the marketers use Advertisement as a tool to attract substantially new customers and to retain the existing customers. This research studies about the â€Å"Influence of Advertisement in Consumer Brand Preference in the Soft Drink Market†, which is one of the most competitive markets in Batticaloa. Every Brand in this market use Advertisement as a major weapon to overcome the fierce Competition. There are numerous Advertisements of different Soft drink brands exposed in Television. But, the main thing here is, the marketer want to identify that, do all these advertisements positively influencing the consumers’ brand preference. In order to study the influence of Advertisement in Consumer Brand Preference, three main variables are considered with appropriate dimensions. They are; Information, Communication and Comprehension. The structured questionnaire was used to collect primary data from 200 respondents. The study found that all three variables indicate high influence of Advertisement in consumer brand preference. Even though it has high influence in overall view, the advertiser wants to consider the indicators, which have low and moderate influence in their future developments of the Advertisements to maintain its position in the market in the long-run. Eventually, this study recommends some actions for improving the influence of Advertisement in consumer brand preference. – 2 – Introduction The importance of sales on business survival and the connection between customers and sales, it is expedient for organizations to engage in programmes that can influence consumers’ decision to purchase its products. This is where advertising and brand management are relevant. Advertising is a subset of promotion mix which is one of the Four ’P’s in the marketing mix i. e. product, price, place and promotion. As a promotional strategy, advertising serve as a major tool in creating product awareness and condition the mind of a potential consumer to take eventual purchase decision. Marketers’ survival depends on consumer satisfaction. Consumer satisfaction depends on their perception and brand preference of the particular brand. In brand preference, advertising plays a major role. Nearly everyone in the modern world influence to some degree by advertising. Organizations in both public and private sectors have learned that the ability to communicate effectively and efficiently with their target audiences is important to their success. In today’s world, there are a myriad of media outlets-print, radio, and television are competing for consumers’ attention. There are number of creative and attractive advertisements we can see and hear in television, Radio, newspapers and in magazines. Within these media, television advertisements are more attractive and interestingly watched by mass audience. It has often been said that television is the ideal advertising medium where the consumer spends the most â€Å"attentive† time. However, the main thing here is, the marketer want to identify the influence of advertising in consumers brand preference. Nowadays soft drinks have become essential part in lifestyle of the people in the society. There are number of soft drink brands are available in the market. In those brands, some brands are very famous not only in Sri Lanka but also globally. For the research purpose Coca-cola, Fanta, Elephant, and Ole are selected. These are the most preferred brand of the consumers in Sri Lanka. For these brands, different advertisements are available in Television. Some brands’ advertisements are more attractive than others are and some are new creative advertisements. There is no any television advertisement regarding Pepsi during the research period. Therefore, Pepsi was not take into consideration. – 3 – It is very hard to find people who never consume any brand of soft drink. Everyone in the society prefers a particular brand. We can see frequent advertisements for Soft drinks in Television. Companies spend much on their advertisements to attract more customers. So it is very important to study its’ influence in consumer brand preference. Problem statement There are numerous advertisements in Medias; television, radio, newspapers and magazines but, the important question for a marketer is â€Å"do all these advertisements positively influence the consumers’ brand preference?If advertisement is not create any positive change in consumers’ brand preference, all the resources such as money, time and efforts spent on advertisement will go in vain.

Serving Customers in Global Markets Essay Example | Topics and Well Written Essays - 1000 words

Serving Customers in Global Markets - Essay Example Laughter, fear, sadness, desire, and inspiration are just some of the many emotions TV induces in viewers. TV reaches millions of people worldwide, is affordable, and combines audio and visual power to create the ultimate advertising machine. Below is a screenshot of a TV advert: Website advertising uses the power of the internet to convey the intended messages to consumers. With increased internet penetration and the expansion of the â€Å"internet generation,’ website advertising is giving TV a run for its money (Aalberts & Nill, 2012:97). In fact, website advertising is currently the second-most powerful advertising media in the world, in terms of revenue and reach. Just like TV, when used correctly, website advertising can trigger different emotions in consumers and capture their attention (Barry, 2012:37). These facts make these two media the best options for discussion in this paper. Below is an image of a website advert: Understanding consumers’ psychology provides marketers and manufacturers with knowledge of consumer preferences and spending habits. The clichà © saying, ‘customer is king† rings true in this case (Proulx & Shepatin, 2012:58). Understanding consumers’ psychology supports selling products or services that are almost guaranteed to be successful. Studies have shown that marketing expenses, marketing risks and failure of products or services are reduced when consumer psychology is understood. The message the advertiser wishes to communicate in the TV advert is that the new song is exclusive, it is affordable, and it comes with other incentives that the viewer will not get elsewhere. In the website advert, Dolan Jewellers wants the consumer to know that they have a moving sale, there are special offers, and time is running out for those who have not bought anything yet. Research has shown that emotional and psychological messages resonate more with customers than feature and

The rise of the German unification and how it affected the french Research Paper

The rise of the German unification and how it affected the french military policy during the period of 1840-1870 - Research Paper Example Germany is a state in Europe that rose into power during the German Unification of 1840-1870, and became a part of the Status Quo. Before it became unified, Germany entered into different wars such as the Austro-Prussian War, Danish War, and Franco-Prussian War. Prussia was a major boost for the German Unification, after it defeated Austria that had dominated Germany. The Unification was not an easy process, but the Germans were patient. Their main strengths were the growth of the economy, the effective railway network, and superior weapons. 1Today, Germany is a modern state, in the 19th Century it was made up of a collection of smaller states that formed the German Confederation. These Confederations were controlled by Austria. In the 1860`s, the control of Austria was challenged by the Prussia, the development of the unification, and codification of Germany. 2The process of unification began as a gradual evolution of economic affinity from the early stages of Industrial Revolution in the mid 19th Century, which made the German state modify towards economic unification. Some of the economic modifications were like the growth of the railway network in Germany, which made it easy to access different resources across the Confederations. This railway network helped to enhance economic growth, and economic prosperity. This growth also resulted to the formation of the Zollverin Customs union; this accordance was amongst the German states, to introduce a preferential custom policy for its members. Schleswig and Holstein were two German duchies that were under the control of the Danish. The Holstein population was largely German speaking and the Schleswig was a mixture of Germans and Danes. 3In the year 1840, the Dane tried to claim Schleswig and Holstein to be part of Denmark, instead of having a semi-independent duchy. This angered the German nationalist and demanded for the two duchies to be incorporated into the German Confederation. In

Privacy is not the most important right Essay Example | Topics and Well Written Essays - 1000 words

Privacy is not the most important right - Essay Example There was very limited control in the society. However, modern institutions have developed controls that have raised very various privacy concerns. Privacy has become a matter of concern to many thinkers, law makers, human rights activists and other essential policy makers. Despite the significance of the right to privacy and the existing laws supporting it, government agencies, security departments and legislative authorities across the globe have engaged in laws and acts that violate the right to privacy as a basic principle that define human dignity. The guiding principle for security agencies in Britain is that security interests and ensuring stable human security is supersedes the basic right to privacy. The United Kingdom has overemphasized the protection of human security at the expense of the constitutional right to privacy. The installation of public surveillance cameras in major cities and towns in Britain is an example of a major violation of the fundamental right to privacy in the country. As a matter of fact, every individual has something to hide from the public, a fact that may be infringed by the installation of surveillance cameras. It is a universally acknowledged that people should not be allowed to hide under the guise of the right privacy to commit crimes and cause harm to law abiding members of the society. Proponents of this point of view hold that privacy is not one of the most fundamental human rights. Most scholars will concur with my position that, privacy defines comprehensively the right to be left alone and refrain from sharing an individual’s private information with other people. Some of the common issues that are catered for by privacy rights include: individual truths, private matters and issues, individual information as well as ones secrecy. The right to privacy is commonly popular and profound to free people. It offers a significant amount of

We were soldiers Essay Example | Topics and Well Written Essays - 750 words

We were soldiers - Essay Example The General and his men were killed at the Little Bighorn Battle. An intuitive leader, Lieutenant Colonel Moore manages to accomplish the unfeasible despite being out-numbered by his enemy; Lt. Col. Moore refuses to surrender and ultimately proves victorious after forcing the Vietnamese troops to leave their headquarters. Lieutenant Colonel Moore uses a variety of supervisory techniques in leading his unit to conquest. The first technique that Lt. Col. Moore applies prior to him and his troops getting to the combat zone; is that of leading through example. A leader earns loyalty and respect after setting example so that others follow. This involves a leader practicing what he or she preaches. The night prior to their departure, Lt. Col. Moore conveys a speech to his unit at a party he had held, where he pledges that although he might not bring all his soldiers alive home; he would be the first to enter the battlefield and the last to leave. He adds that, dead or alive, he would not leave any person behind. He makes sure that each of his men is not left at the battlefield after the battle is over, true to what he had promised. During war, Lt. Col. Moore also leads through example many times; for instance, he refuses to surrender and performs other acts of heroism. The second supervisory strategy that is obvious in Lt. Col. Moore’s leadership is that of treating everyone with dignity and respect. In the same message delivered by Lt. Col. Moore to his men, he factors out that every man in his unit, despite his origin or race, is an essential participant of the troop. â€Å"†¦here in the States some men in this unit may experience discrimination because of race or creed, but for you and me now, all that is gone (Wallace n.pg).† By guaranteeing that everyone recognized this, Lt. Col. Moore was building assurance, self-respect and team cohesiveness that would go a long way in guaranteeing that his unit members trusted each other. Finally, Lt. Col. Moore is

An Analytical Remuneration of Writing Skills Personal Statement - 5

An Analytical Remuneration of Writing Skills - Personal Statement Example Initially, it was an intimidating experience filled with apprehensions and anxieties. However, the encouragement provided by a highly competent and qualified instructor prodded me to endeavor to enhance my writing skills. Through observing grammatical rules and by indicating weaknesses in structure and form, I had begun to improve in terms of gaining confidence in writing and in applying the rules for effective writing. In this regard, it is my personal conviction that I am given the privilege to pass the UWSK course as a prelude to more gratifying writing experiences in the future. The UWSK accorded me with the opportunity to develop diverse skills pertinent to writing. One of the major skills learned was an observance of essay structures which encompasses important rules in grammar, including observance of correct spelling and punctuation marks. My initial essay (Out of Class Essay #1) on Illegal Immigration observed appropriate structure by presenting an introduction (which could actually be fairly long), a body (with arguments presenting claims and reasons) and an appropriate conclusion. I took ample time to clearly evaluate correct spelling and phrasing with the use of the required punctuation marks. I realize that there is still a tendency for me to lengthen my discussion of a particular topic creating the tendency to divert into another topic. Of course, one cannot be expected to come up with a perfect essay in a few tries. Another skill that has been developed through the UWSK course is the skill of introspection – the ability to contemplate on one’s thoughts and feelings prior to writing. As indicated in the standard instructions prior to any writing experience, one must brainstorm to gather thoughts, information, details of what I want to write, and how I plan to write these ideas in a structure which would assist the reader in understanding the message that I want to relay.

Visual Analysis Essay Example | Topics and Well Written Essays - 750 words

Visual Analysis - Essay Example However, a tiger soon mauls him as they wander about the jungle, leaving the woman alone. The initial parts of the storyline depict a confused woman left alone too face all the horrors of the jungle. The directors employ visuals to take the audience through the stages of struggles for survival up to a point where the woman realizes the power within her to be the master of the jungle. As such, one can conduct a visual analysis of the music video using thesis statement that human beings are the masters of their environments as long as they are ready to overcome their challenges. A visual analysis of the music video confirms this assertion as shall be indicated below. To begin with, a visual analysis of the music video reveals a lot about the woman and her interaction with the jungle. The directors used color, shape and line to bring out her turmoil. As one may see (Roar 00:00:55), a visual analysis of the music video depicts the woman as scared, confused and as in torment. The form and shape of her face reveals a reaction to something scary. She is either screaming or completely disoriented about something. Use shape and form also reveals that spiders are attacking her. This gives her creeps and sends cold chills up her spine. One may also observe that the use of color and background light have also been employed to bring out the situation in this context as Patterson (48) observes in visual analysis. Use of fading dark colors in the background confirms that she is out by herself in the jungle at night. Such an understanding enables the audience to put themselves in her situation and possibly comprehend her reactions. It also serves as a precursor to the later development in other scenes. Color and light are very instrumental in sending a message about the sub-theme depicted, which is fear on this case. Such visuals are critical for various analyses as Block (79) indicates. However, she starts to be acquainted with the world around her as depicted in this caption . In this scene, one may observe that the woman is now starting to be acquainted with the jungle. At scene 00:02:27, a visual analysis of the music video now depicts the woman in a different way. The directors have contrasted this scene with the previous ones where the woman was in total shock and fear. The use of warm colors with cool green colors in the background now brings out the woman as quite comfortable in her environment. She has made clothing from leaves and other branches. Shape has been used to bring out a calm and confident face while color values and tone confirm that she is better than before. She comes out as an emerging champion as far as adapting to an environment is concerned. He apparent ease with herself and confidence as she swings on a presumably huge hanging root of a tree may be taken to imply her progress in mastering her environment. Another visual feature that the music video employs is space. Although the woman in the music video has mastered her environ ment quite well, the reality is that she is all alone with the animals in the jungle. She wishes to be home. When she stand on a branch and sends a shout-out to her animal friends, one may say that she is also trying to find out if some help could be forthcoming (Roar 00:02:40). A visual analysis reveals an empty space on her sides and in front of her. This is the space of separation after the air crash that has seen her as the only survivor in the thick jungle. Like in many visual depictions, the directors have

Price Elasticity of Demand Essay Example | Topics and Well Written Essays - 1000 words

Price Elasticity of Demand - Essay Example With changes in market dynamics, burgeoning population, increasing competition in job markets, and rapid technological changes that out phase odd manual jobs, demand for formal education has skyrocketed over the last decade. This happens as people strive to better their competitiveness in securing formal employment. Placing the  high value on academic certificates as an essential aspect in securing any employment has led to an increased demand for further education across  the  board, which in turn creates high demand for colleges and universities, giving way to the proliferation of many institutions offering bachelor degrees and masters as well. A study on income levels among employees with different academic qualifications reveals that there are wide disparities in salaries of diploma, the  degree, and masters degree holders. Salaries increase with  the  increase in academic qualifications with master’s degree holders earning twice as much as degree holders who i n turn earn twice what high school diploma holders earn on average. Those with no formal education form the bulk of unemployed population across the country, often performing poorly paid manual jobs in homesteads, municipal councils and mining zones among other similar places. Office jobs are a preserve for the highly qualified in  the formal education, especially those with bachelor degrees and above. Management jobs are common with master’s degree holders and those with many certificates in support courses.... Other factors that affect the elasticity of demand include changes in income levels, persistence of price changes, and product demand patterns among others. With changes in market dynamics, burgeoning population, increasing competition in job markets, and rapid technological changes that out phase odd manual jobs, demand for formal education has skyrocketed over the last decade. This happens as people strive to better their competitiveness in securing formal employment. Placing high value on academic certificates as an essential aspect in securing any employment has led to an increased demand for further education across board, which in turn creates high demand for colleges and universities, giving way to the proliferation of many institutions offering bachelor degrees and masters as well. A study on income levels among employees with different academic qualifications reveals that there are wide disparities in salaries of diploma, degree and masters degree holders. Salaries increase with increase in academic qualifications with master’s degree holders earning twice as much as degree holders who in turn earn twice what high school diploma holders earn on average. Those with no formal education form the bulk of unemployed population across the country, often performing poorly paid manual jobs in homesteads, municipal councils and mining zones among other similar places. Office jobs are a preserve for the highly qualified in formal education, especially those with bachelor degrees and above. Management jobs are common with master’s degree holders and those with many certificates in support courses relevant to their field of occupation. This state of affairs places high value

Employment Labor and Anti-Discrimination Law Essay Example for Free

Employment Labor and Anti-Discrimination Law Essay You are an employee at a nice paper mill and want to impress the boss by burning the midnight oil (cut not too close to the wood pulp). For four consecutive weeks, you work forty-five, forty-two, thirty-nine, and thirty-one hours. If you are a nonexempt employee covered by the Fair Labor Standards Act, how many hours of overtime pay, if any, would you be entitled to? What would be the rate? Fair Labor Standards Act Fair Labor Standards Act (FLSA) of 1938 is concerned with child labor, maximum hours, minimum wages, and overtime provisions. Overtime An employee who works more than forty hours over week must be paid 1.5 times his or her regular pay rate for all hours over forty. Employers whose jobs are categorized as executive, administration, or professional, as well as outside salespersons and computer employees, are exempt. Employees who are not exempt include manual laborers and other blue-collar workers who perform tasks involving repetitive operation with their hands (non-management production line employees, for example). Other non-exempt employees include police officers, firefighters, licensed nurses and other public safety workers. Employers can pay overtime to exempt employers if they want to, but they cannot otherwise waive or reduce the overtime requirements. The employee will be paid overtime for the weeks he or she worked the forty-five, forty-two, but not the thirty-nine or the thirty-one hour week.

The Great Gatsby Essay Example for Free

The Great Gatsby Essay In American society, the way people act is quite an interesting, yet confusing subject to look at. If you were to look closely at the behavior and the thinking of the average American man in the modern day, you would see that he is not too different from a man that lived one hundred years ago in America. Obviously many things have changed in society that make a man different nowadays compared to one hundred years ago, but the point is that, in general, the mind of an American person has kept the same characteristics. A great way to understand how an American mans mind has remained the same is by comparing The Great Gatsby with modern society in the United States. In this novel, which takes place in the 1920s, we see that the major themes incorporated into it are hope in the American Dream, the idea that rich are always entitled and never responsible for their behavior, and that the common man will keep trying to achieve the American Dream to the fullest even though he is vaguely aware that he has higher morals than the person he is seeking to become. Ever since the United States was founded, man has always hoped of becoming successful and even prosperous. All men were created equal according to our Declaration of Independence so the American Dream is something that any man can achieve if he puts in the work and the dedication needed. Plenty of hope is also needed if a man wishes to become successful in the United States because sometimes hard work and dedication alone are not enough. The main character in The Great Gatsby, Jay Gatsby, has, â€Å"some heightened sensitivity to the promises of life,† which is like saying that Gatsby is an average man that devotes his life to the American Dream and getting what he wants. Gatsby also has, â€Å"an extraordinary gift for hope,† which means that Gatsby has a positive attitude toward achieving the American Dream. Jay Gatsby can relate to many success stories that have happened recently in the United States. One example of a modern day Jay Gatsby is Oprah Winfrey. Oprah was born into a poor life with not much opportunity, but she did have hope and eventually she was able to work her way to the top of the American Dream. Having hope when trying to become successful is a must or your dream may never happen. It seems as though the old money, rich people think they have entitlement no matter what they do. The wealthy can have whatever they like all the time even if they dont morally deserve it. This has been the case in America for a long time, including the time of Jay Gatsby and also in modern day society. A major character in The Great Gatsby, Nick Carraway, realizes that the rich lack responsibility for their actions and they continue living as though they have every right to their good fortune. Nick says, â€Å"They were careless people, Tom and Daisy – they smashed up things and creatures and then retreated back into their money or their vast carelessness, or whatever it was that kept them together, and let other people clean up the mess they had made. † Even when Daisy murders Myrtle, Gatsby ends up paying the price for her because Daisy and Tom are careless and rich. Money is way more important to them than anything else in the world. There are many people in our society today like Daisy and Tom. One example is Paris Hilton, who basically goes around and parties with her fathers money. She can do whatever she wants and the money is always there to cover it up. It is amazing how money can determine someones character. The common man seems to always fight to have the American Dream even if they realize that they have higher morals than those who already are successful. Money can blind a person from realizing that having good morals is more important than having financial success. The Great Gatsby shows that Jay Gatsby innocently pursued the American Dream, he worked on personal improvement his whole life, and he continued his hard pursuit even with forces working against him. He ultimately was killed because of people unlike him that had money but no morals. Gatsby tried to have money and great morals and that is something that is not exactly possible. People nowadays try all the time to pursue the American Dream while remaining a good person with morals. It is hard to have it both ways and that is evident after reading about Jay Gatsby. Realizing that modern day society is still the same as it was in The Great Gatsby is important. The author, F. Scott Fitzgerald, would very likely agree that todays society is no different than the society portrayed in his novel. Everybody wants to pursue the American Dream and remain as a good person, but as you look at Jay Gatsby, it is easy to see that it is extremely hard to accomplish and maintain. Maybe soon society will become a fair playing ground, but for now all people can do is hope that life works out for the better.

Effects of Static and Dynamic Culture Conditions

Effects of Static and Dynamic Culture Conditions Tissue engineering has been investigating the properties of scaffolds and cell culture conditions for better cell attachment, viability and proliferation. This study compares the two cell culture conditions: Static and spinner flask / dynamic cell conditions over a period of 7 days on polyglycolyic acid. The scaffolds were statistically seeded by mouse dermal 3T3 fibroblast in static culture method and on other hand seeded scaffolds were transferred to spinner flask at approx.60 rpm in dynamic culture method. Significant improvement in cell viability was not observed in both the conditions after 7 days of culturing. The cells adhesion successfully took place and expressed cytoskeleton ÃŽÂ ²-actin in both the methods but achieving maximum distribution of cells on the scaffold in dynamic method. This study reports that static culture method could produce increase in cell number approximately six times more after 7 days of culture i.e. from 1.2 x 10à ¢Ã‚ Ã‚ µ ( ±0.1610à ¢Ã‚ Ã‚ µ) cells to 6.3 x10à ¢Ã‚ Ã‚ µ ( ±110à ¢Ã‚ Ã‚ µ) cells. Surprisingly, instead of enhancing the growth of 3T3 fibroblast cells in dynamic condition, they seems to be probably undergoing cell death/loss as reported by alamar blue, hoechst DNA assays, toludine blue and western blot. Overall, static condition favoured the cell adhesion, proliferation and ÃŽÂ ²-actin expression gradually with days and produced better reproducible data compared to dynamic condition. The techniques involved in dynamic culture method needs to be more carefully investigated and improved further to draw a strong conclusion. The aim of the study is to implement the principles of fundamental techniques in tissue engineering in culture method on the three dimensional polyglycolic acid (PGA) scaffolds seeded with 3T3 fibroblast. To compare and contrast the effects on cells in spinner flask or dynamic culture condition method with the static culture condition method by observing and analysing on factors like cell adhesion, distribution, proliferation, viability and expression of cytoskeleton after culturing in the same system for 7 days using alamar blue, hoechst 33258 DNA assays, toludine blue staining and western blot analysis. Tissue engineering is a multidisciplinary field which aims in developing new approaches for functional substitutes applicable in restoration of damaged or injured tissues. These substitutes are complex constructs of living cells, bioactive molecules and three dimensional porous scaffolds, which supports cell attachment, proliferation and differentiation. Therefore, its main objective to achieve in therapy is to form a living tissue from small population of mammalian cells. For this, the ideal tissue engineering strategy so far has remained to develop tissue by seeding the specific population of cells on three-dimensional constructs which not only provides a structural support to cell mass but also can effectively influence cell attachment, growth and differentiation either by incorporation of adhesion molecules or controlled release on bioactive molecules from the scaffold. After seeding of cells onto the 3-D scaffolds construct, the cells starts proliferating which results in deposi tion of extracellular matrix components and biodegradation of scaffolds. The latter makes the porous construct of scaffold more solid 3-D. Several other factors affect the 3D tissue growth including scaffold design, seeding method and the culture condition methods. Studies have reported that high degree of cell attachment to biocompatible and biodegradable particles, while avoiding aggregate formation can be achieved using poly co-glycolic acid (PGA) scaffold of 50-100mm spherical size fabricated by electro-spinning technique. This method provides reliable, reproducible and well-characterized PGA scaffold. The surface chemistry of the scaffold helps to determine the particle size, shape, morphology and distribution. Depending on the experiments, surface modifications are performed like formation of poly l-lactide-coglycolide (PLGA) via ring opening polymerisation and fibronectin coating to scaffolds. However, it is not the part of standard protocol. Depending on the size, the required cell density for maximum attachment may differ to obtained optimal cell attachment. The seeding is usually done using the cell suspension of a particular seeding density which allows for maximum dispersion of cells and well integration into the pores of the scaffolds. But for therapeutic purposes, however, this strategy is not sufficient enough to result in an overall improvement in conditions due to severe tissue damages. This can be overcome only by achieving relatively high degree of cell attachment to the micro-particle. Several factors and parameters influence the cell adhesion like the curvature of the particles, the particle material, the electrostatic charge of the particles, the surface motif of the particles, the interaction between cell and particles, the number of cells in the tissue culture and type of cell culture method implemented. It is also important to obtain homogenous cell adhesion to the scaffolds and avoiding clumping which will lead to the formation of cell-particle aggregates. This will prevent cells from appropriate uptake of nutrient from the media and hinder their subsequent growth. The mammalian cells are usually cultured in static or bioreactors condition. Here in this study, spinner flask system is employed which is also a kind of bioreactor as it provides the 3D environment. It is a flask provided with magnetic rod which keeps rotating constantly at specified speed. The nature of growing cells requires such dynamic condition to mimic the environment similar inside the body which gives sufficient nutrient supply, waste exchange, enhances ECM and gap junction formation, and cell-cell interaction. Most importantly it also helps maintain the cells differentiated in 3D which is needed for tissue formation. This characteristic is not maintained by static culture method. Hence, many 3D culture methods have been developed such as perfusion chambers, rotary vessels and commercial perfused bioreactors with improved capacity for mass transport of nutrients and waste product. They help in formation of relatively good quality of tissue by more enhanced cell differentiati on and also maintaining in that state. The static culture method used in this study, tissue culture plastic with seeded scaffolds remains untouched in the incubator. But with static culture, alternative shaking on a shaker and resting can also be employed to provide better supply of nutrient through media. The attachment characteristic of ECM proteins such as laminin, will also depend upon the cell type used. There are particular conditions needed to be optimised with each cell type. Most of the tissue engineering experiments uses 3T3 fibroblast only to optimise the cell culture condition where there is optimum cell adhesion is obtained before using the actual stem cell of interest. This is because, 3T3 fibroblast are known to easily attach to any surfaces due to presence of the high density of integrins on their cell surface. This will not only enhance the cell attachment but will also give maximum possible interaction with the particle. Cells that have spread on the particles exhibit a clear halo of cytoplasm surrounding their nucleus after the rearrangement of their actin skeleton. The attachment and spreading of cells to a substrate surface is often seen as a basic characteristic, but is, in fact, the initial process that subsequently influences and regulates cell growth, survival, migration and differentiation. In addition, cell-to-substrate interaction, mediated by integrins, also influence cell behaviour and signalling pathways leading to modifications in upstream and/or downstream cellular activity. Thus, a desirable substrate should allow sufficient and optimal cell attachment and spreading characteristics to occur. The 3T3 fibroblast media is used in which DMEM supplemented with 10% FCS enhances the cell attachment as the serum is highly protein rich and therefore, helps in cell in adhesion by supplying the ECM-proteins as well as nourishing them. Hence, the serum conditioning step is of critical importance in maintaining cells health and attachment in the culture. Materials and Methods Scaffold preparation and serum conditioning PGA FELT Scaffolds disc of 2mm x 10mm and 45mg/cc (TE005-50-10) was provided by Smith and Nephew research group, University of Nottingham. These non-culture scaffolds were then treated in 24 well tissue culture plastics (TCP) plates with 3T3 fibroblast media containing 500ml DMEM (Sigma G7513) supplemented with 10% FCS, 2mM L-glutamine and 1% AB/AM (Sigma A5955). All the scaffolds were statistically seeded on day 1 using non-culture treated well plates to encourage the cells of mouse dermal 3T3 fibroblast to adhere to the scaffolds at seeding density of 1x 10à ¢Ã‚ Ã‚ ¶cells/ml. 3T3 fibroblast cell suspension was added in TCP plates for all test and no cells in the blanks. The plates are then incubated overnight at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ in air. The remaining cell suspension was then again resuspended in warm media to achieve 4 x 10à ¢Ã‚ Ã‚ ¶ cells/ml cell density and was stored at -20 °C till day 7 for Hoechst analysis. 3T3 fibroblast cells were used to seed the scaffolds to observe the cell viability, cell proliferation and ÃŽÂ ²-actin expression on day 1, when the cell culture condition was maintained static and day 7, after applying the two cell culture conditions (static dynamic) and maintaining for 7 days. Static culture In static culture condition, the seeded and non-seeded (blanks) scaffolds were kept in 1ml of warm 3T3 fibroblast media per well. These five culture plates were kept in incubator and cultured for 7 days at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ in air. Spinner flask culture Two separate spinner flask filled with 50ml warm media each was used for seeded scaffolds and non-seeded (blanks) scaffolds. These flasks were kept in incubator by loosening the side arms and setting the magnetic stirrer approximately at 60rpm and cultured for 7 days at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ in air. After following 7 days for culture conditions, the construct was then sacrificed for alamar blue, toludine blue and Hoechst analysis. Also, in addition cytoskeleton analysis using western blot was also carried out. The assessment of two culture methods, static and dynamic was done by producing five set of readings for static condition and four set of readings for dynamic condition where the experimental analysis were conducted using three replicates for test and blanks on day1 and day7 Alamar Blue Assay Staining was done using 10% alamar blue containing 1ml alamar blue (Serotec BUF012B) and 9ml HBSS without phenol red (Sigma H1387). The stain was kept in dark at 37 °C. The scaffold was transferred from seeding and culture conditions to new 24-TCP non-cultured plate with 1ml warm alamar blue after washing three times with PBS. The plates were then incubated at 37 °C, 5%COà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡ for 1hr. The aliquots of 3 x 100 µl of alamar blue were transferred to 3 wells of 96 microtitre well plate including the blanks to measure fluorescence using plate reader (Ex530nm/Em590nm). The excess of alamar blue solution was aspirated and washed with 1ml sterile PBS. Toludine Blue Staining Scaffold for toludine blue staining was transferred to new non-culture treated 24well TCP plate and was treated with 1ml ice cold 95% (v/v) methanol in dHà ¢Ã¢â‚¬Å¡Ã¢â‚¬Å¡O for 5 mins after washing 3 times with 1ml warm PBS. Then fixative was discarded and scaffold was allowed to air dry at RT followed by treatment with 1ml aqueous 0.1% (w/v) toludine blue (Fisher chemicals BPE107-10) for 5 mins. The scaffold was again allowed to air dry at RT. Papain Digest and Hoechst 33258 DNA Assay The aliquot of cell pellet (4 x 10à ¢Ã‚ Ã‚ ¶ cells) prepared on day1 was treated with 1ml of papain solution (1.06mg/ml, pH 6.5) (Sigma P4762) followed by overnight incubation in waterbath at 60 °C. The serial dilutions of the papain digested cell pellet using hydrolysed papain solution as diluent was prepared for 4.0, 2.0, 1.0, 0.5, 0.25, 0.125, 0.0625, 0.0312 and 0 x 10à ¢Ã‚ Ã‚ ¶ cells. In the Hoechst 33258 DNA assay, the hydrolysed papain solution was used as blank. 5 µl of each aliquots + 70  µl Hoechst dilution buffer was added in triplicates in black 96-well plate including the blank. In each well, 100  µl Hoechst 33258 working solution (Sigma S6639) was also added and fluorescence was measured using plate reader (Ex 360nm/Em 460 nm) Western Blot 100 µl cold RIPA buffer (Sigma R0278) was added to the cell pellet (4 x 10à ¢Ã‚ Ã‚ ¶ cells) and the seeded scaffolds in eppendorf from day 1 and was kept on ice for 20 mins while vortexing every 5 mins. The cells were then snap freezed by placing it on dry ice for 1 min then 1-3 min at RT. The cells are resuspended by grating and spinning for 30 mins. The supernatant was used for western blot. 10 µl of molecular weight marker and each sample were loaded onto SDS polyacrylamide gel. The electrophoresis was carried out for 90 mins at 125V. After SDS-page electrophoresis, the filter paper, nitrocellulose and sponge were soak in transfer buffer (Invitrogen NP0006) with 20% (v/v) methanol. The assembled western blot tank was run for 1 hr at 25V. The immune-detection of protein ÃŽÂ ²-actin was performed using primary antibody anti-mouse ÃŽÂ ²-actin (Sigma A2006) and secondary antibody anti-mouse horse radish peroxidises (HRP) (Invitrogen G21234). Statistical analysis All the data obtained was calculated using MS-Excel spreadsheet and statistic Independent t-test and paired t-test analysis was performed using SPSS software. Results and Discussion Morphology of 3T3 fibroblast cells The cell of mouse dermal 3T3 fibroblast was obtained from T180 flask by trypsin digest method is shown in figure1. The flask was confluent enough (80%) and morphology of the cells seems to be intact and healthy. No sign of contamination was observed prior to seeding procedure. The morphology of 3T3 fibroblast cells are of flat and spindled shape. These cells form a well-characterised and established mesh like interconnected networks. This property of fibroblast cells make them ideal for cell attachment as they show anchorage property due to presence of integrins in ECM. Hence, using this cell type achieving maximum cell adhesion onto the scaffolds becomes ideal for this experiment. Effect on Cell viability in static and dynamic conditions The alamar blue assay was performed on the static and dynamic culture condition to observe its effect on 3T3 fibroblast cell viability is shown in figure 2a and 2b. The culture method employed aims to maintain or increase the cell viability when cultured for seven days. Under static condition (Fig 2a), only 1 group out of five showed significant increase in fluorescence whereas other two groups showed more or less no change in their fluorescence produced from day 1 to day 7. Also, on contrary two groups showed significant decrease in fluorescence on day 7 (Fig 2a). Hence, variable of results were obtained between groups. On the other hand, under dynamic condition, the cell produced more fluorescence on day 7 compared to day1 expect for one group. Therefore on an average, when mean of the static absorbance reading was taken, it showed that there is significant decrease in fluorescence (fig 2b). But in dynamic method, the increase in fluorescence day 7 (Fig 2b) was not significant enou gh. The 3-D construct of PGA scaffold provides with an environment to the cells where they remain viable in culture for several weeks. Moreover, they should successfully increase the cell viability after some days. However, our study reported that the cell viability decreased tremendously in cell seeded PGA scaffolds in static cell culture condition whereas the dynamic cell culture method was able to increase the cell viability over 7 days of culture. So, when comparing the two culture methods statistically showed difference in their overall effect on the viability of 3T3 fibroblast cells where dynamic condition is more but not effective enough. So, static condition did not improve the cell viability more than dynamic culture method. Effect on Cell distribution in PGA scaffolds The three-dimensional PGA scaffolds constructs enables the fibroblastic cells to adhere and to evenly distribute throughout the porous structure. To assess the uniform 3T3 fibroblast cell distribution in two different culture conditions, toludine blue staining was carried out on day 1 and day 7 on both conditions is shown in fig 3. Toludine blue stains cell dark blue within the 3-D construct. As observed in static condition, on day 1 the cells were successfully seeded onto the scaffold but compared to day 7 the cells are not evenly distributed throughout the scaffold. Also, the scaffolds were efficiently seeded on day1 under dynamic condition as the figure 3c shows cells stained with toludine blue. Surprisingly, on day 7 (Fig 3d), the scaffolds shows no cells at all. This means, that the 3T3 fibroblast cells under dynamic condition was eventually lost or died. The spinner flask culture system might have loose the cells by day 7 due to poor adhesion or vigorous rotation. The cell seed ed on day 1 was too low or error in carryout the technique. But this was observed with all the spinner flask condition system, where the success was 2 out of 4 groups (Supplementary data 3). However, this observation is more of debate because no other factors expect the condition itself could affect cell distribution as uniform distribution was achieved in all the five static condition (supplementary data 3) which used the same scaffolds and cell type. Effect on Cell proliferation in static and dynamic conditions 3T3 fibroblast was culture over 7 days in both conditions to also observe its effect on the cell proliferation are shown in figure 4 (a, b, c d). The standard curve obtained with known cell density for both static and dynamic of all the groups (fig 4a 4b) showed increase in cell density with increase in the fluorescence. The unknown cell density of the cells from these two culture methods on day 1 and day 7 was calculated and found that 2 out 4 groups from dynamic conditions had no cells in the culture on day 7. Therefore, only other two groups were considered to evaluate the cell number on day 1 and day 7. There was significant difference in cell density over 7 days of culture in static method (n=5)(fig 4c) and on contrast there was no significant difference in cell density in dynamic method (n=2)(fig 4c). Almost all the groups showed cell density on day 1 around 1 x10à ¢Ã‚ Ã‚ µ cells/ml which was the actual cell density seeded on day 1 (supplementary data 4). This shows that se eding performed on scaffold achieved effective adhesion of all the cells present. The mean cell number from 1.2 x 10à ¢Ã‚ Ã‚ µ ( ± 0.16 x 10à ¢Ã‚ Ã‚ µ) cells on day 1 increased to 6.3 x 10à ¢Ã‚ Ã‚ µ ( ± 1 x 10à ¢Ã‚ Ã‚ µ) cells on day 7 under static culture method (fig 4d). On the other hand, dynamic culture methods showed hardly any change in cell number over 7 days of culture i.e. 2.0 x 10à ¢Ã‚ Ã‚ µ ( ± 0.92 x 10à ¢Ã‚ Ã‚ µ) cells on day 1 to 2.5 x 10à ¢Ã‚ Ã‚ µ ( ± 1.96 x 10à ¢Ã‚ Ã‚ µ) cells on day 7 (fig 4d). Previous studies have reported using other cell types that they start proliferating within 24 hrs after seeding cells on scaffolds employing dynamic culture methods. Contradicting this, our results have shown that dynamic had really poor effect on cell proliferation. Moreover, 3T3 fibroblast cells were undergoing death during seven days of culture. Whereas, static culture method shows drastic increment in the cell number and thus supporting 3T3 fibroblast cell proliferation efficiently. The scaffolds used for alama r blue assay on day 1 were used for Hoechst DNA assay with same after washing step (same for day 7 scaffolds). The washing might have been too vigorous which resulted in cell loss. It could also be possible that cells are being aspirated off from the culture which gave poor or no cell proliferation. It should be also taken into account that the success rate with dynamic culture method on cell proliferation was null out of 4 demonstrations. Expression of Cytoskeleton For the analysis of expression of cytoskeleton ÃŽÂ ²-actin on 3T3 fibroblast in two different conditions was done by western blot as shown in figure 5. The cell pellet of density 4 x10à ¢Ã‚ Ã‚ ¶ cells/ml was loaded against the cells obtained on day1 and day 7 from static and spinner flask culture method. The density of ÃŽÂ ²-actin obtained from the cell pellet was maximum. The amount of ÃŽÂ ²-actin detected on day 1 was lower than day 7 in static culture condition. It was the opposite scenario with spinner flak method where day 7 had minimum amount of ÃŽÂ ²-actin compared to day1. In some cases of spinner flask method ÃŽÂ ²-actin was not even detected on day 7 (supplementary data 5). Hence, comparatively the expression of ÃŽÂ ²-actin was higher in static culture method. Perhaps, it could be because the cell could not proliferate much as expected. Also, the culture didnt have enough cells left to express ÃŽÂ ²-actin on western blot. The formation of ECM cytoskel eton was not shown to be supported by spinner flask method. Conclusion and future work The tissue engineering scaffolds constructs have been shown more effective on cells containing serum in spinner flask/dynamic culture method rather than in static culture method. But from our data, it shows that dynamic condition only favoured cell adhesion and distribution. It was also able to produce a small increment in cell viability unlike static culture method. Contradicting the other data, cells were virtually not detected on day 7 and so is the expression of ÃŽÂ ²-actin. Not only this, all the 4 demonstration failed to show that cell growth can be effectively supported in dynamic culture method. Three seeded scaffolds were kept in spinner flask together, where there is increased chance for it to come in contact with each other. Cells may get detached from the scaffolds as it might be loosely adhere to the scaffold. The continuous rotation of magnetic rod in the flask circulates the media to provide nutrients to cells more effectively then static. Despite of this fact, the cells were either undergoing cell death or dislodged from the scaffolds or may be aspirated off from the culture. The static culture method have been effective in 3T3 fibroblast adhesion on the construct after seeding and eventually could improve tremendous cell growth by showing increase in cell proliferation over a period of seven days in culture. However, better distribution and increased ÃŽÂ ²-actin expression could only be achieved by the static culture method after 7 days as the cells proliferated more. Moreover, the success rate for this method was more compared to dynamic and produced more reliable and reproducible data. Hence, it can be concluded that static culture method supported cell growth better then the dynamic culture method. It would be interesting to investigate the technique involved in dynamic culture method more carefully to produce reliable data where it could be compared with the static method to give better understanding of the environment cells require to grow in artificial ECM-like structure and culture media. Since, within the body the cells are continuously under the force by blood flow in 3D environment, it would be useful to derive cell culture growth better in dynamic condition with enhanced technique. It is strongly recommended to carry out further research in this area to conclude spinner flask methods effect on 3T3-fibroblast cells with more reliable data. Evaluation The practical session assessed my learning in the techniques and concepts involved in tissue engineering. The demonstration on different techniques to prepare scaffolds assessed my understanding better and was helpful to apply same in this practical session by evaluating the different parameters that can be influenced by the scaffold design alone. As earlier discussed troubleshoot, implementing the technique given in protocol helped to produce the good replicates and contamination free-blanks and controls. While working in the hood with the partner, things were discussed prior to carrying out the experiment and working space was kept ready which helped in managing the use of same equipments, solution and incubation time effectively to avoid any source of contamination. Also, the exchange of results and data between several groups also led to the exchange of ideas and different cause for their results. However, the exact reason for spinner flask method to not work out is still not cle ar as all the groups got same reading where cells were present onto the scaffolds during alamar blue assay on day1 and day 7 but eventually lost when subsequent assays were done for same day. Overall, the difference between the effects of two culture method was evaluated. Acknowledgement The efforts put in by the Paula Ellis is acknowledged was carryout the change of media and taking care for the samples throughout the practical session and also during weekends. Also, Dr. Felicity Rose for giving the guidance and helping with doubt regarding the techniques and protocol. The images and data taken from all other groups are acknowledged for sharing their data used in this report. The effort of the group member is also acknowledged for managing with the working protocol load effectively. Figures Figure1. 3T3 fibroblast cells in T180 flask (10X). The image shows morphology of 3T3 fibroblast prior the trypsin digest followed by static seeding. The image was taken using Nikon (Scale bar: 80 µm) Figure 2a. Alamar blue assay for all static (n=5) and for dynamic (n=4) culture methods on day1 and day 7. The graph shows fluorescence detected  ± SD for both the culture condition. The absorbance value of non-seeded scaffold (control, Ac) was subtracted from the absorbance value obtained for seeded scaffold (As) to optimise the calculated fluorescence i.e. As-Ac. This was done for all the static and dynamic culture methods data. The statistical analysis paired t-test was at 95% significance level was done using SPSS. The calculated data is provided in the supplementary data. Figure 2b. Alamar blue assay of static and dynamic condition on day 1 and day 7. The mean of all the values on day 1 and day 7 for static (n=5) as well as dynamic (n=4) was done. The graphs shows the mean of absorbance (O.D)  ± SD. The statistical analysis was performed using paired t-test and independent t-test at 95% significance level. DAY 1 DAY 7 Figure 3. Toludine blue assay. The toludine blue staining was performed on static culture condition on day 1 (a) and on day 7 (b). Similarly for dynamic culture condition on day 1 (c) and day 7 (d) was carried out. In (a) and (b) there is darker background staining but (c) shows proper stained 3T3 fibroblast cells. No cells staining can be detected in (d) (Scale bar: 100 µm). Figure 4a. Standard curve for all static condition using Hoechst 33258 DNA assay. The standard curve was plotted using the known concentration 4.0, 2.0, 1.0, 0.5, 0.25, 0.125, 0.0625, 0.0312 and 0 x 10à ¢Ã‚ Ã‚ ¶ (blank) of 3T3 fibroblast cells against the absorbance obtained. The blank was subtracted from the test reading to standardise the graph. The graph was produced using MS-Excel, to obtain the linear regression and linear equation for each group to calculate the cell density in static culture condition. Figure 4b. Standard curve for only two dynamic condition using Hoechst 33258 DNA assay. The standard curve was plotted using the known concentration of 4.0, 2.0, 1.0, 0.5, 0.25, 0.125, 0.0625, 0.0312 and 0 x 10à ¢Ã‚ Ã‚ ¶ (blank) of 3T3 fibroblast cells against the absorbance obtained. The blank was subtracted from the test reading to standardise the graph. The graph was produced using MS-Excel, to obtain the linear regression and linear equation to calculate the cell density in dynamic culture condition on day 1 and day 7. Figure 4 c. Hoechst 33258 DNA assay was carried out on all static (n=5) and dynamic (n=2) culture condition on day 1 and day 7. The cell density was calculated using the standard curve for its own respective group. The graph shows cell density (x 10à ¢Ã‚ Ã‚ µ cells/ml)  ± SD for static and dynamic condition. The calculation was performed on excel-sheet and statistical analysis of paired t-test was done using SPSS. Figure 4 d. Hoechst 33258 DNA assay. The unknown cell density calculated from standard curve was averaged (mean) for static (n=5) and dynamic (n=2) culture methods. The graph shows cell density (x 10à ¢Ã‚ Ã‚ µ cells/ml)  ± SD for static and dynamic condition. The calculation was performed on excel-sheet and statistical analysis of paired t-test and independent t-test was done were appropriate using SPSS. Figure 5. Western blot analysis of 3T3 fibroblast cell from static and dynamic on day 1 and day 7. The expression of ÃŽÂ ²-actin in both culture methods are analysed using the rainbow marker and compared with the actual pellet of 3T3 fibroblast to cells extracted from two different culture methods on different days.

Essay --

Did you know that over one million people injure themselves each year because of slipping and falling? 300,000 people are injured each year from slipping and falling in the workplace and 15% of workplace deaths are due to the injuries caused by slipping and falling. Slips and falls can be easily prevented by the store or business with anti slip treatments, securing electrical cords, removing rugs or mats or other tripping hazards, routine maintenance, and cleaning up spills as soon as they happen. There are many reasons as to why you could slip and fall and those causes and factors include: †¢ Water †¢ Ice †¢ Snow †¢ Grease or oil †¢ Objects in the way †¢ Floor cleaner †¢ Uneven surfaces †¢ Areas with lots of foot traffic †¢ Unstable areas †¢ Ladders †¢ Stairs †¢ Areas prone to wetness or spills †¢ Bad lighting If you do slip and fall you should ask yourself the following: †¢ What caused the slip and fall? †¢ If the floor was slippery, why was it slippery? †¢ Was it more slippery then normal? †¢ Was there any warning that the floor was slippery? †¢ Did the owner or landlord know abo...

The Dual Role of Gods in The Iliad Essay -- Iliad essays

The Dual Role of Gods in The Iliad      Ã‚   With even a cursory exposure to ancient Greek texts, it is obvious that the gods and goddesses are very important in traditional Greek culture. As literary figures in mythos and specific poetry and drama, the gods dabble in the life of man, predict his fate, and routinely thwart any attempt for him to entirely forge his own future. But for those of us who are not extensively schooled in antiquities, it is hard to pinpoint exactly what the gods are to the ancient Greeks, and what they are to us as readers of literature who live outside the culture. Were the gods accepted as parable figures, meant to instruct? Were they used to explain acts of nature? Do they now belong to anything outside the scope of literary history?    Rather than speculate about the role of gods in all of Greek culture, it is more manageable to look at one specific text and determine the role its gods play within its world. In The Iliad, the gods are an integral part of the poem. Their foibles and fickleness recall for the reader the humanness of the Greek gods, and spark a mental association of men to myths. This makes the long-dead warriors more real to anyone who reads the poem. But the gods of The Iliad also inculcate what could be nothing more than a dry account of a historical war that no one recorded while it was happening. This historical-cultural element, one that connects the events of that unwritten war to readers by pulling the past into the present, make the old archetypes oddly modern and applicable to the present day world and its men. One of the most interesting lines in The Iliad is when one Aias tells the other that he recognizes Poseidon, who has disguised himself as K... ...ormalized remembrance; the gods' inclusion make that remembrance bigger than any sterile account or battlefield casualty list could be. This expanded scope makes relevant the deaths of would-be anonymous warriors, makes tragedy out of widows and orphans, makes us think about the cycles of human aggression. The gods and their powerful presence is one element of this relevant piece of historic art.    Works Cited and Consulted Camps, W. A. An Introduction to Homer. Oxford: Clarendon Press, 1980. Homer. "The Iliad." Western Literature in a World Context: The Ancient World through the Renaissance. Ed. Paul Davis et al. vol 1. New York: St. Martin's Press, 1995. 25-156. Steiner, George, and Fagles, Robert, eds. Homer: A Collection of Critical Essays. Twentieth Century Views, ed. Maynard Mack. Englewood Cliffs, N. J.: Prentice Hall, 1962.   

Aborigines and their Place In Politics :: social issues

Aborigines and their Place In Politics For much of their history, Australia ¡Ã‚ ¦s major parties did not perceive a need to have  ¡Ã‚ ¥Aboriginal affairs ¡Ã‚ ¦ policies, but this altered in the 1960s and 1970s as the Aboriginal interest came to occupy a more prominent position. The policies of recent major governments, those being the Australian Labor Party (ALP) and the Coalition, consisting of the Liberal Party and National Party, have changed drastically since the Federation of Australia. The approaches throughout history of these major parties will be discussed briefly in order to gain an understanding of the foundation of each party ¡Ã‚ ¦s beliefs and platforms in regards to Aborigines. The main political issues facing Aborigines in society today will be identified, and subsequently the main political parties approach and policies will be distinguished in relation to each issue. Finally, recent policies and legislation introduced by the main political parties will be introduced and discussed. From 1937, the approach of all governments was one of  ¡Ã‚ ¥assimilation ¡Ã‚ ¦, whereby Aborigines would submit to indoctrination in white ways before taking their place in the general Australian community. However, in time this policy came under intensifying attack on all sides, with critics claiming the policy denied these individuals of their Aboriginal culture, and enforced the notion of the superiority of the white culture. For a time,  ¡Ã‚ ¥integration ¡Ã‚ ¦ became a policy of the Commonwealth, though it was hard to identify the distinction between  ¡Ã‚ ¥assimilation ¡Ã‚ ¦ and  ¡Ã‚ ¥integration ¡Ã‚ ¦. As attitudes changed, State governments began to amend many of the laws that denied Aborigines equality with whites. In 1967, all parties maintained the proposed Constitutional amendment. Although attitudes had begun to change, little had been done to encroach such altered attitudes in definite government policies. The Labor Party made the most positive pitch for these interests, and at its 1971 Federal Conference, Gough Whitlam led the party into conceiving the most detailed Aboriginal affairs policy yet adopted up until this period, by a major party. This called for the establishment of a full Aboriginal affairs department. Whitlam guaranteed that a Labor government would not falter to override any State laws  ¡Ã‚ ¥which discriminated against Aborigines, or which supervised Aborigines, or which reduced the opportunities for Aborigines to conduct themselves as they wished ¡Ã‚ ¦. Shifting aside  ¡Ã‚ ¥assimilation ¡Ã‚ ¦ and  ¡Ã‚ ¥integration ¡Ã‚ ¦, Labor adopted  ¡Ã‚ ¥self-determination ¡Ã‚ ¦, a policy which spoke of Aborigines ultimately being able to  ¡Ã‚ ¥decide the pace and nature of their future development ¡Ã‚ ¦, where they would  ¡Ã‚ ¥take a real and effective responsibility for their own affairs ¡Ã ‚ ¦.

Documentation of Billing System Essay

Brief description of the proposed thesis topic The word ‘billing’ can refer to both the total amount of goods and the act of counting them. Others take a billing to insure the number of items ordered matches the actual number of items counted physically. Statement of the Objectives General Objective The general objective is to create a computerized billing system for J&S Oyster Restaurant. Specific Objectives The following are the specific objectives of the study that will determine the deliverables: * To present an accurate computation on the bill charged to the customers. * To produce a print out on every sales on a daily weekly monthly basis. * To secure records on the database. * To procure a more accurate orders eliminating errors done manually. * To facilitate a faster transactions in billing the customers. Significance of the proposed thesis topic or justification This study was made to make the work easier, faster and more accurate in terms of billing. The proposed system was developed and implemented to lessen the work of management of the company. It provides more accurate and reliable records of billing (day, week and month). Review of Related Literature and Studies Ritchie, Marshall and Eardley (1998) mentioned that by providing a suitable support environment, office automation system are intended to benefit the knowledge workers in two days: Direct benefits are better control over work, due to less division of labor and fewer non-productive activities such as filling, record keeping and updating. Another benefit would be indirect benefits, are less quantifiable, and may enrich the organization through long-term profitability and growth. These benefits can be less dependency on other department for support with copying printing and similar activities, less need for procedures and control to monitor work flow between departments, increased individual job satisfaction due to greater personal effectiveness in carrying out the range of tasks, greater customer satisfaction, due to better information production and a more timely service, increase competitiveness of an organization through the improved use of its information resource and its ability to respond to business pressures on opportunities. IT applications have changed the nature of the workplace. Tasks ranging from taking orders to analyzing business plans are done using computers rather than paper and pencil. Where people once relied done wherever and whenever it is most convenient. Alter (2001) cited that using telecommunications technology as a substitute for travel is one part of a trend of bringing the work to the workers rather than the workers to the work. In the same general vein, the phrase †any place any time† increasingly summaries the customer’s expectation that business will provide what the customers wants, when and where the customer’s wants it. With the fast and ever changing technology, communication plays a vital role in any business as this will allow its client and the company to interact at an instant. Greater connectivity supports the continuing convergence of computing and communication whereby communication capabilities have become essentials to many systems, and computing capabilities have become essential to communication systems. Consider the way sales people at many firms use touch-one telephones to obtain pricing information and enter orders. In these systems, the telephone becomes a data entry terminal for an information system. (Alter 2001) Since the endeavor will use SMS technology in providing the automated inquiry service, population over the texting market will undeniably needed. Olive (June 16,2004) in his article said the, the Philippines was cited recently as Asia’s leader in short message service by CommunicAsia 2004, an annual telecommunication and broadcasting held recently in Singapore. He further stated that there are already 25 million mobile subscribers. This phenomenal growth can also be equated to the affordable and accessible mobile service. At this time, SMS has become more popular and is not limited to personal usage; different sectors of the population are now using this technology to gain competitive edge in information technology. Nowadays, the use of SMS technology great helps us in our daily lives, most of the time we communicate with others through SMS. The proposed system allows the use of SMS technology for notification feature that sends message to the MIS head when a computer peripheral is altered.

Beliefs About Students Essay

It is undeniable that the beliefs about students and education have a profound impact on what is taught and how it is taught, just as the beliefs about life have a profound impact on how individuals live their lives. Educational beliefs are revised and refined and over time becomes stronger as they seem to serve us well and prove to be true. Thus, these beliefs ultimately become our philosophy of education. It is possible that every student that enters a classroom can succeed. However, in order for them to accomplish this, teaching should be student centered. Students should be encouraged to understand and appreciate their uniqueness and to be accountable for their learning and behavior. According to Ornstein and Hunkins (2004) Perennialism relies on the past; especially the past asserted by agreed-on universal knowledge and cherished values. Dunn (2005) also agreed that Perennialism is the oldest educational philosophy and is therefore traditional. It is believed that students are vessels to be filled and disciplined in the proven strategies of the past. This philosophy is supported by the realist philosopher John Locke, who was of the belief that at birth the mind is a blank sheet of paper on which the teacher could write knowledge (Tabula Rasa). According to the essentialist viewpoint, there are certain basic or essential knowledge, skills and understandings that students should master in order to function successfully in the society. These are reading, writing, computing and in today’s world, computer skills. Plato, who was the father of idealism, believed that both male and female are equal and should be educated equally. Therefore, the curriculum is the same for everyone and planning to execution of lessons are dominated by the teacher. However, one should not forget that these beliefs are teacher centered and tend to be more authoritarian and conservative and emphasize only the values and knowledge that have survived through time. Learning occurs through experience, Therefore students should be allowed to develop cognitively through experimentation and to reach their own personal potential instead of being shaped into a specific mould. Dunn (2005) cited Immanuel Kant who agreed that knowledge is rooted in the experience of mankind and can only be achieved by an active and exploratory mind, rather than one that is passive and receptive. Carl Rogers also believed in experiential learning. He believed that unlike academic knowledge, experiential knowledge is required to meet the needs of the learner in order to complete important real-life tasks such as learning to drive a car. Every student is different, no single set of learning outcomes is appropriate for all students. Thus a set curriculum will not be suitable for all. Students should be able to determine what they need to study with the guidance of the teacher. This will help them to arrive at their own understandings. Jean Sartre, a leader of existentialism believed that man’s responsibility is vested in man himself. He further stated that people are entitled to be human with dignity andis a human only when he or she is entirely free and accepts responsibility for his freedom. You may be led to believe that students are blank slates and learn based on mainly academic knowledge that has been tried, tested and proven and that all humans are equal and should be educated equally. However, do not ignore the fact that students are taking to the classroom, their own ideas and materials rather than receiving material in class as it is given. Students restructure the new information to fit into their own cognitive frameworks. In this manner, they actively and individually construct their own knowledge. They also are different thus, the same rigorous curriculum will not work for all students. From these facts one may conclude that in order to help students reach their full potential and gain the knowledge and skills that they will require in their daily lives as a democratic citizen of our society, the student centered approach to how students learn must be practiced. Therefore it is recommended that teachers facilitate students instead of being fountains of wisdom. The teacher should help students in their pursuit for knowledge and also help them acquire the communication, problem solving and critical thinking skills which will enable them to be life-long learners.

Parenting Skills Essay

1. What role do you think discipline plays in developing a child’s self-esteem? What forms of discipline best serve the self-esteem of the child or adolescent? 2. List and discuss how activities, clubs, or sports, impact the self-esteem of children and parents. Provide at least two activities, clubs, or sports in your answer. Discussion Answers 1. I think that discipline done correctly could help in a child’s self-esteem. When you discipline a child make sure that they understand WHY and it makes them feel trustworthy, and included. If you treat discipline like a dictatorship the child feels like you’re abusive and they are stupid and untrustworthy. people with low self-esteem generally Fail to achieve their goals, Are not able to pursue accomplishments in a responsible and disciplined manner, Have poor communication skills, Have a pessimistic view on life, Are prone to anxiety, depression, hostility, loneliness, shame, and guilt, Form unhealthy, destructive relationships with others, and Make decisions in life more to please others than to adhere to their own interests and value systems. I think that discipline done correctly could help in a child’s self-esteem. When you discipline a child make sure that they need to understand what is happening and they need to feel included. If you treat discipline like a dictatorship the child feels like you’re abusive. people with low self-esteem generally fail to achieve their goals, have poor communication skills, have a negative view on life and are prone to anxiety, depression, loneliness, and guilt. Also, form unhealthy, destructive relationships with others, and make decisions in life more to please others. 2. Parents should encourage children to participate in sports and clubs that are productive and give a feeling of accomplishment. However, it is not always advantageous for a parent to push a child toward a particular sport or activity because sometimes the parents will push their children towards their dream sports or activities they wanted to do as a child. 3. parents should encourage children to participate in sports and clubs that are productive and give their children a feeling of accomplishment. However, it is not always advantageous for a parent to push a child toward a particular sport or activi ty.

Proposal for Quality of Work Life Essay

INTRODUCTION Human resources play a very important role in success of an organization and thus, management of human resource assumes importance. Many aspects affect the management of human resources. One such aspect is Quality of Work Life (QWL). It is a philosophy, a set of principles, which holds that people are the most important resource in the organization as they are trustworthy, responsible and capable of making valuable contribution and they should be treated with dignity and respect . The elements that are relevant to an individual’s quality of work life include the task, the physical work environment, social environment within the organization, Administrative system and relationship between life on and off the job. QWL consists of Opportunities for active involvement in group working arrangements or problem solving that are of mutual benefit to employees or employers, based on labor management cooperation. People also conceive of QWL as a set of methods, such as autonomous work groups, job enrichment, and high involvement aimed at boosting the satisfaction and productivity of workers. It requires employee commitment to the organization and an environment in which this commitment can flourish. Thus, QWL is a comprehensive construct that includes an individual’s job related wellbeing and the extent to which work experiences are rewarding, fulfilling and devoid of stress and other negative personal consequences. According to Gadon (1984), QWL programs have two objectives: (a) to enhance productivity and (b) to increase the satisfaction of employees. Thus QWL provides healthier, satisfied and productive employees, which in turn provides efficient and profitable organization. Quality of work life (QWL) is viewed as an alternative to the control approach of managing people. The QWL approach considers people as an ‘asset’ to the organization rather than as ‘costs’. It believes that people perform better when they are allowed to participate in managing their work and make decisions. This approach motivates people by satisfying not only their economic needs but also their social and psychological ones. To satisfy the new generation workforce, organizations need to Concentrate on job designs and organization  of work. Further, today’s workforce is realizing the Importance of relationships and is trying to strike a balance between career and personal lives. Successful organizations support and provide facilities to their people to help them to balance the scales. In this process, organizations are coming up with new and innovative ideas to improve the quality of work and quality of work life of every individual in the organization. Various programs like flex time, alternative work schedules, compressed work weeks, telecommuting etc., are being adopted by these organizations. Technological advances further help organizations to implement these programs successfully. Organizations are enjoying the fruits of implementing QWL programs in the form of increased productivity, and an efficient, satisfied, and committed workforce which aims to achieve organizational objectives. The future work world will also have more women entrepreneurs and they will encourage and adopt QWL programs. Whilst there has, for many years, been much research into job satisfaction (1), and, more recently, an interest has arisen into the broader concepts of stress and subjective well-being (2), the precise nature of the relationship between these concepts has still been little explored. Stress at work is often considered in isolation, wherein it is assessed on the basis that attention to an individual’s stress management skills or the sources of stress will prove to provide a good enough basis for effective intervention. Alternatively, job satisfaction may be assessed, so that action can be taken which will enhance an individual’s performance. Somewhere in all this, there is often an awareness of the greater context, whereupon the home-work context is considered, for example, and other factors, such as an individual’s personal characteristics, and the broader economic or cultural climate, might be seen as relevant. In this context, subjective well-being is seen as drawing upon both work and non-work aspects of life. However, more complex models of an individual’s experience in the workplace often appear to be set aside in an Endeavour to simplify the process of trying to measuring â€Å"stress† or some similarly apparently discrete entity. It may be, however, that the consideration of the bigger, more complex picture is essential, if targeted, effective action is to be taken to address quality of working life or any of it’s sub-components in such a way as to produce  real benefits, be they for the individual or the organization. Whilst Quality of Life has been more widely studied (4), Quality of working life, remains relatively unexplored and unexplained. A review of the literature reveals relatively little on quality of working life. Where quality of working life has been explored, writers differ in their views on its’ core constituents. It is argued that the whole is greater than the sum of the parts as regards Quality of working Life, and, therefore, the failure to attend to the bigger picture may lead to the failure of interventions which tackle only one aspect. A clearer understanding of the inter-relationship of the various facets of quality of working life offers the opportunity for improved analysis of cause and effect in the workplace†¦.This consideration of Quality of working Life as the greater context for various factors in the workplace, such as job satisfaction and stress, may offer opportunity for more cost-effective interventions in the workplace. The effective targeting of stress reduction, for example, may otherwise prove a hopeless task for employers pressured to take action to meet governmental requirements. OBJECTIVES OF THE STUDY GENERAL OBJECTIVES * To study the level of satisfaction of employees towards the quality of work life. * To study the importance given by the organization to quality of work life * To study suitable measures to improve the quality of work life. * To identify the major areas of dissatisfaction if any, and provide valuable suggestions Improving the employee’s satisfaction in those areas. * To study the role and importance of good quality of work life of employees in an organization SPECIFIC OBJECTIVES: * To study the present and overall quality of work life in the  organization. * To understand the relationship between QWL and employee satisfaction. * To study quality of work life contributed for employees personal and professional development STATEMENT OF THE PROBLEM The major factor which has influenced the increasing importance of provision of quality of work life is employees themselves. Workers are changing. They have become more educated and independent. Close attention to QWL provides a more humanized work environment. Hence it is very essential that every organization ensure that their employees have a positive quality of work life. Quality of Work Life in an organization is essential for the smooth running and success of its employees. The quality of work life must be maintained effectively to ensure that all employees are running at their peak potential and free from stress and strain. The Quality of Work Life can affect such things as employees ‘timings, his or her work output, his or her available leaves, etc. Quality of Work Life helps the employees to feel secure and like they are being thought of and cared for by the organization in which they work. An organization‘s HR department assumes responsibility for the effective running of the Quality of Work Life for their employees. This being the real fact and since there was absenteeism and lack of job satisfaction among the workers in an organization, the researcher has made an attempt in this regard and has undertaken the current study to analyze the Quality of Work Life in this organization and to offer suitable suggestions for the organization to take necessary steps to improve the Quality of Work Life among its Workers. NEED OF THE STUDY There is a much about the quality every aspect viz. quality product, quality of material and inp uts there in, quality of packing, quality of product development and quality of service. The quality of work life and quality of life, which is pivotal aspects in everyone’s work life. This also brings employee satisfaction You can obtain man’s physical presence at a given place, and a measured number of skilled muscular motions per hour or day. But the enthusiasm, initiative, joy, loyalty, you can’t obtain by devotion  of hearts, mind and souls. Apart from this if the employee is provided with other extrinsic and intrinsic benefits then this will lead for high productivity and results in employee satisfaction too. To introduce the hard practices in to the organization it is the important to have encouraging atmosphere. QWL is one of the most important factors, which leads to such favorable atmosphere. It produces more humanized jobs. It attempts to serve the higher order needs of employees are human resources that are to be developed rather than simply used QWL leads to an atmosphere that encourages than to improve their skill. It also leads to have good interpersonal relations and highly motivated employees who strive for their development. QWL will ensure enthusiasm work environment with opportunities for every one to give is best. Such job will provide job satisfaction and pride to the company. DEFINITIONS CONCEPTUAL DEFINITION * According to R.E.WALTON(1973) â€Å"Quality of work life is a process by which an organization responds to the employees needs for developing mechanisms to allow them to share fully in making the decisions the design their lives at work† * According to THOMAS S. BATEMAN AND SCOTTA.SNELL(2003) â€Å"Quality of work life refers to programmes designed to create a workplace that enhances employee well-being† OPERATIONAL DEFINITION * â€Å"Quality of work life defined as the level of employee’s satisfaction or dissatisfaction on   Working conditions at the work place† * â€Å"Quality of work life can be define as â€Å"The quality of relationship between employees and the total working environment† RESEARCH DESIGN Descriptive research design – This is used â€Å"to describe systematically the facts and Characteristics of a given population or area of interest, factually and accurately† One of the main benefits of descriptive research is that fact that it uses both quantitative And qualitative data in order to find the solution to whatever is being studied. This in turn can help to describe and give an answer to certain life experiences. For example, when carrying out a case study descriptive research allows various sources to be Considered such as personal accounts. With this in mind, it enables a wider view of an issue As opposed to strict numbers and figures which can only account on facts rather than Experiences. UNIVERSE For the purpose of this study the employees may belong to any manufacturing company in Chennai. SAMPLE SIZE In this study the sample size is 50 SAMPLING METHOD Non Probability Sampling: Non Probability sampling refers to methods of selecting individuals to include in a study where some elements of the population have no chance of selection, or where the probability of selection can’t be accurately determined. Non Probability sampling methods include accidental sampling, quota sampling and purposive sampling. SAMPLING TECHNIQUE PURPOSIVE SAMPLING. Purposive sampling starts with a purpose in mind and the sample is thus selected to include people of interest and exclude those who do not suit the purpose. This method is popular with newspapers and magazines which want to make a particular point. This is also true for marketing researchers who are seeking support for their product. They typically start with people in the street, first approaching only ‘likely suspects’ and then starting with questions that reject people who do not suit. The universe is very large and the sample size is relatively small (50). The samples to be taken  Purposively from manufacturing sector. Purposive sampling technique facilitates data collection on the basis of availability of the samples and convenience of the researcher. METHODS OF DATA COLLECTION Essentially two types PRIMARY DATA AND SECONDARY DATA Primary data are those which are collected for the first time and are original in character. Data which are originally collected by the investigators are called primary data while the secondary data are collected through some other sources. For example, information collected by an investigator from a. student regarding his class, caste, family background, etc., is called primary data. On the other hand, if the same information collected about the student from the school record and register, then it is called secondary data. TOOLS OF DATA COLLECTION The tool to be used to collect data here is the interview schedule. INTERVIEW SCHEDULE An interview schedule is a list of questions to ask the interviewee. It should start with more open questions. An interview schedule is prepared to effectively find out useful and accurate information. CONCLUSION This research proposal serves a small outline about the future project on the topic Quality of work life. This is the current emerging problem that most of the sectors Are working towards to improve it.

Character and Setting in Popular Music

Whether through characters and scenes music in popular music or written literature like novels, a character can convey emotions and thoughts. The audience understands through the present role. Just as we find the character we like, we also find characters that look down. The setting is almost the same. Whether explicitly given or implicitly set, the audience will think about the picture. Sometimes it's a beautiful place, sometimes it's a place we'd rather ignore. In this article, we will explain the role of the environment in public machinery. Analyze the relationship between the central figure and the surrounding environment. Do you set character limit options or affect plot development? Does the environment play an important role in raising the theme of the story? Popular Machinery was written to imagine details to the audience. Men and women in the story are anonymous, there is no clue to the explanation of the story, there is no mention of the city, state or country where the sto ry takes place. This allows the reader to draw the context and detail of the story in his own way. Perhaps the reader has heard of it or is in a similar situation and you can put your face and place in the story. The setup details are very simple, but it is also the most detailed information on every element of the story. Whether through characters and scenes music in popular music or written literature like novels, a character can convey emotions and thoughts. The audience understands through the present role. Just as we find the character we like, we also find characters that look down. The setting is almost the same. Whether explicitly given or implicitly set, the audience will think about the picture. - Setting is a term indicating where the behavior occurred in the narrative work and the past time. However, the settings can be used for other purposes of the novel, such as as a symbol supporting the novel theme. Various settings of red letters such as market, forest, Hester Cott age are examples of this. The market is a typical department of a tough Puritans society In psychology literature, the word personality has become common. This is a term popular in the early 20 th century. Back then, people believed that people had a particular character and had a series of virtues and moral personality traits, including integrity, honesty, generosity, reliability. But by the middle of the century this word became unpopular and rarely used. The main reason for being rejected by psychologists is that according to the mainstream theory at the time, human behavior is determined not by individual but by environment.

Finance project Assignment Example | Topics and Well Written Essays - 1000 words

Finance project - Assignment Example An analysis of the financial forecasts presented gives an insight into the financial requirements of the project. Suitable methods of project evaluation such as Net Present Value method, Internal rate of Return and Profitability Index approaches can be used to evaluate this project. Decision making by the Board of Directors can be based on one, two or all of the above methods. Determination of the cost of capital is significant as this will give the discounting rate to be used to discount the cash flows expected from the project. A rate of 8 percent is determined using the dividend growth rate model. This rate is adjusted to incorporate the risk factor present in the business. The risk adjusted discounting rate is therefore 11 percent. Depreciation is calculated using the MACRS approach of General Depreciation System. The rates are as prescribed in Table A-1 (US Treasury 946). A depreciation tax shield is calculated based on the depreciation values and this is added back as a benefit. The NPV approach gives a Net Present Value of $ 20,893,000. Therefore, the project should be implemented as it promises a positive NPV. Financially, the project is viable. If the funds obtained were non-interest bearing, the project would be financially viable under this approach. Under the Internal Rate of Return method, the PV of all future incomes are compared against the Initial investment and the discounting rate which equates the two represents the IRR. It is that discounting rate which yield a zero NPV. From the financial data obtained, the rate is determined as 11.48%. This is above the investors’ required rate of 8% and the risk adjusted rate of 11%. The project therefore will generate a higher return and should therefore be implemented. Using the Profitability index approach, the PI of the project is more than 1 and therefore viable. It yields a PI of 1.16. The project should therefore be implemented on this

Input and out put for the computer Essay Example | Topics and Well Written Essays - 250 words - 1

Input and out put for the computer - Essay Example Most prevalent input devices are the keyboard and the mouse. Keyboard is similar to the typewriter, it helps in typing in text, numbers, symbols, punctuation, letters etc. Pressing an enter key, sends the information to the processing device. Mouse is a pointing or clicking device that helps in locating and selecting the files at a faster pace. It has three buttons the left button helps in selecting the file or the folder or the icon on the desktop, a right button which when clicked displays the properties related to the file, while the middle is to scroll the page. Other input devices are scanner, light pen, joystick to play games, digital camera etc. The result or the outcome after the processing could be displayed by the output devices. The most prevalent output device is the monitor, it is like a TV screen and also called as VDU (Visual Display Unit). Whatever is typed through the keyboard can be seen on the monitor screen. The results of the input are being displayed on the monitor. This is called the soft copy as we can only read it and store the results. When the computer is switched off, results could not be retrieved therefore hard copy or the printouts are taken by another output device, the printer. Other input/output devices are the Floppy disk, CD-ROM, Pen drives etc. They also act as portable storage devices as they store the information from the computer and also provide the information to the